Front Inner Page - Volume 5 No.3 June 2018

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  • Title

    :

    SILICA-COLLAGEN COMPOSITE (B30) PERFORMED BETTER AS SCAFFOLD BIOMATERIAL THAN ITS MODIFICATION IN VITRO

    Authors

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    Chernet Woyimo Woju (Researcher)

    Keywords

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    Cell adhesion, composite, Equine Mesenchymal Stem cells (EqMSCs), tissue engineering, Bone.

    Issue Date

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    June 2018

    Abstract

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    For treatments of connective tissue disorders in both human and veterinary medicine, culturing of adult stem cell in combination with biomaterials such as natural biopolymers holds great promise. However, the safety and performance of such therapies relies on the systematic in vitro evaluation of stem cellbiomaterial constructs prior to in vivo implantation. This study evaluated B30 (70% silica+ 30% collagen) and B30Sr20 (50% silica+ 30% collagen, 20% strontium) composite polymer as biomaterials for their application in bone tissue engineering in veterinary medicine under pellet and shear stress culture conditions. The qualities of biomaterials were measured by analyzing cell adhesion using DAPI staining and cell proliferation by MTT assay. Live cell imaging for cell viability (cell migration) after plating of the materials with cells in culture dishes were done using florescence microscopy. Cell morphology on the biomaterials was investigated using scanning electron microscopy (SEM). Furthermore, osteogenic differentiations were tested by driving the cells into the osteogenic lineage using specific growth and differentiation factors. Osteogenic differentiation were analysed by detecting typical morphological changes, by specific staining procedures called Alizarin red method as well as using qualitative PCR. The biomaterials characterized using SEM demonstrated that only B30 induced fiber bundling of cells thus B30 were shown to be cytocompatible, supporting cellular adhesion, and allowed for osteogenic differentiation of the cells under pellet culture condition than fluid shear stress culture. No significant differences were observed in proliferation potential for cells seeded either with B30 or B30Sr20 in both culture conditions. Generally, it could be concluded that cells on the B30 scaffold were viable and metabolically more active than B30Sr20 and culturing the MSCs with B30 under pellet culture condition has shown more promising results than in fluid shear stress. These findings demonstrate that the combination of a B30 (silica-collagen-xerogels) and MSCs are promising constructs for bone tissue engineering applications

    Page(s)

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    1-8

    ISSN

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    2347- 4734

    Source

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    Vol. 5, No.3, June 2018

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